rabbit polyclonal antibodies against cgrp  (Cosmo Bio USA)

Journal: bioRxiv

Article Title: Metastatic infiltration of nervous tissue and periosteal nerve sprouting in multiple myeloma induced bone pain

doi: 10.1101/2022.12.29.522199

Figure Lengend Snippet: (A, B) Effect of anti-netrin-1 treatment (NP137 10 mg/kg, i.p.; dosing days represented by arrows) or vehicle on sham or MM mice. (B) Post-surgical day 26 limb use score of sham or MM mice treated with vehicle or NP137. *p<0.05 by one-way ANOVA followed by Holm-Šídak post-hoc test. (C) Survival plot of NP137 treated mice. (D) Endpoint spleen weight. ****p<0.0001 by one-way ANOVA followed by Holm-Šídak post-hoc test. (E) Effect of systemic NP137 treatment on bone osteolysis, measured as bone volume per total volume (BV/TV). *p<0.05 by one-way ANOVA followed by Holm-Šídak post-hoc test. (F) Representative images of periosteal CGRP + nerve fibres and GFP + MM cells in sham and MM mice treated with vehicle or anti-netrin-1 treatment. (G) Quantification of CGRP + periosteal nerve density. *p<0.05 by one-way ANOVA followed by Holm-Šídak post-hoc test. (H) Number of vehicle- or NP137-treated MM mice presenting 5TGM1-GFP MM cell infiltration to the periosteum. MM= Multiple myeloma. Sham n=3-4; MM n= 5-10.

Article Snippet: Subsequently, slides were placed in vertical chambers (Shandon, Sequenza Immunostaining, Fisher Scientific; #73-310-017), washed, blocked (3% normal donkey serum, 0.3% triton X-100 in PBS) for 2 h RT and incubated o/n with a primary antibody cocktail containing anti-GFP antibody (polyclonal chicken anti-GFP, 1:2000; #A10262, AB_2534023; Thermo Fisher Scientific, Rockford, IL, USA), anti-TH antibody (TH polyclonal rabbit anti-mouse; 1:1000, Millipore; #AB152, AB_390204), and an antibody against calcitonin gene-related peptide (CGRP; polyclonal rabbit anti-rat, 1:5000; #C8198, AB_259091; Sigma-Aldrich, St. Louis, MO, USA) or growth associated protein-43 (GAP43; rabbit anti-GAP43; 1:1000; #AB5220, AB_2107282; Millipore, Billerica, MA, USA).

Techniques:

Journal: Clinical and Experimental Dental Research

Article Title: Analysis of the mylohyoid nerve in elderly Japanese cadavers for dental implant surgery

doi: 10.1002/cre2.341

Figure Lengend Snippet: View of the reflection of the fascia of the mylohyoid muscle (MM) on the inner surface of the human mandibular body showing CGRP‐positive immunohistochemical staining in a whole mount sample. (a) The origin of the muscle fibers was seen by peeling off the fascia of the MM from the mylohyoid line of the inner surface of the mandibular body (MB). The sites (squares, b–d) are shown in the CGRP reaction areas in (a). The CGRP‐positive sites are found on some fine nerves of the fascia of the MM. The CGRP‐positive sites (arrows) were also mainly located in the large and small vessels (b–d). (b) In the posterior surface region of the mandibular body, small clusters of positive reactions (arrows) were concentrated in the middle zone of the fascia of the MM. (c) In the middle surface region of the mandibular body, small fine fibers that were positive for CGRP (arrows) were found on the fascia of the MM. (d) In the anterior surface region of the mandibular body, small fine fibers that were positive for CGRP (arrows) were found on the fascia of the MM. Ant, anterior region; ILS, inner lingual surface; Post, posterior region. Bar = 1 mm

Article Snippet: These sections were then incubated with rabbit polyclonal antibodies against CGRP (10 μg/mL; ENZO BML‐CA1134) or normal rabbit IgG as a negative control for CGRP at 4°C overnight.

Techniques: Immunohistochemical staining, Staining

Journal: Clinical and Experimental Dental Research

Article Title: Analysis of the mylohyoid nerve in elderly Japanese cadavers for dental implant surgery

doi: 10.1002/cre2.341

Figure Lengend Snippet: The localization of CGRP in immunohistochemically stained sections of the inferior lingual mucous membrane of the mylohyoid muscle (MM). Strongly labeled CGRP‐positive cells (arrows) were found in the mucosal lamina propria beneath the basal layer of the mucosal epithelium of the anterior and posterior regions of the mucosa of the MM (a,b). Fine positive fibers (arrows) were observed around the blood vessels in the anterior region of the mucosa of the MM (c). Small positive cells (arrows) were identified around mandibular glands and muscle fibers and in the fiber bundle of the nerves of the posterior region of the mucosa of the MM (d–f). (a) Anterior region of the mucosal epithelium, bar = 40 μm; (b) posterior region of the mucosal epithelium, the red dotted line indicates the border between the epithelial mucosa layer (EM) and the mucosal lamina propria layer (MLP), bar = 50 μm; (c) anterior region of the mucosal lamina propria, bar = 50 μm; (d) posterior region of the muscle fibers beneath the mucosal lamina propria, bar = 100 μm; (e) posterior region of the submandibular glands beneath the mucosal lamina propria, bar = 100 μm; (f) posterior region of the nerve bundles beneath the mucosal lamina propria, bar = 50 μm

Article Snippet: These sections were then incubated with rabbit polyclonal antibodies against CGRP (10 μg/mL; ENZO BML‐CA1134) or normal rabbit IgG as a negative control for CGRP at 4°C overnight.

Techniques: Staining, Membrane, Labeling

Journal: PLoS ONE

Article Title: Repeated hapten exposure induces persistent tactile sensitivity in mice modeling localized provoked vulvodynia

doi: 10.1371/journal.pone.0169672

Figure Lengend Snippet: (A) Density of CGRP + nerve fibers in 10 μm labiar skin cryo-sections from sensitized mice challenged with Ox or EtOH, displayed as mean ± SEM (n = 3-5/treatment group). Dashed line corresponds to average CGRP + nerve fiber density/μm 2 in untreated mice. Images are representative from day 21 after cessation of challenges (B-D; 20x magnification; scale bar represents 50 μm). Means compared to Ox/EtOH (** = p<0.01, *** = p<0.001) or untreated controls (### = p<0.001) at each time point; significance determined by one-way ANOVA and Tukey Kramer post hoc analysis. (E) Relative abundance of Ngf in Ox- vs. EtOH-challenged mice 1 day after 10 challenges displayed as mean ± SEM (n = 5-6/treatment group; two independent experiments).

Article Snippet: These were fixed and stained with a primary rabbit polyclonal antibody against calcitonin gene related peptide (CGRP; Abbiotec, San Diego, CA; 1:500) and AlexaFluor 488-conjugated secondary antibody (Thermo Fisher Scientific, Wilmington, DE; 1:1000) as previously described [ , ].

Techniques:

Journal: PLoS ONE

Article Title: Repeated hapten exposure induces persistent tactile sensitivity in mice modeling localized provoked vulvodynia

doi: 10.1371/journal.pone.0169672

Figure Lengend Snippet: Density of Avidin + mast cells (A) and CGRP + cutaneous nerves (D) on day 9 after 4 treatments with c48/80 or saline (administered on days 5–8 after cessation of 10 Ox challenges) in 10 μm labiar cryo-sections, displayed as mean ± SEM (n = 2-3/treatment group). Representative images for mast cells (B-C) and nerves (E-F); 20x magnification; scale bar represents 50 μm. Means are compared to Ox/EtOH (** = p<0.01, *** = p<0.001); significance determined using one-way ANOVA and Tukey Kramer post hoc analysis. (G) Tactile sensitivity in mice treated with either saline or c48/80 (n = 6–9 mice per treatment group; two independent experiments). Means are compared to Ox/Ox/Saline (* = p<0.05, ** = p<0.01, *** = p<0.001); significance determined using an unpaired Student’s T test at each time point.

Article Snippet: These were fixed and stained with a primary rabbit polyclonal antibody against calcitonin gene related peptide (CGRP; Abbiotec, San Diego, CA; 1:500) and AlexaFluor 488-conjugated secondary antibody (Thermo Fisher Scientific, Wilmington, DE; 1:1000) as previously described [ , ].

Techniques: Avidin-Biotin Assay, Saline